1st Summer School on InfraRed Nanospectro-imaging 2025

9 Jun 2025, 09:00 → 13 Jun 2025, 16:00 Europe/Warsaw

Institute of Nuclear Physics Polish Academy of Sciences

The Summer School is dedicated to students and young scientists interested in using infrared spectroscopy (IR) to address biological challenges at the nanoscale. The combination of IR with atomic force microscopy (AFM-IR) breaks the diffraction limit and complements the topographic images with information about the biochemical composition.

During the program, participants will gain hands-on experience in the entire workflow, including cell culture, preparation, and measurement using both conventional FT-IR and AFM-IR techniques. Practical experiments will be complemented by lectures from renowned specialists in photothermal nanospectroscopy, such as Professor Alexandre Dazzi, the inventor of AFM-IR, from the Institut de Chimie Physique, Université Paris-Saclay, Orsay, France.

This Summer School offers an exceptional opportunity to work closely with AFM-IR experts who apply this technique across various scientific fields.

Monday 9 June

14:30 → 15:00 Registration & Welcome reception

15:00 → 15:30 Prof. Wojciech M. Kwiatek: "Our dreams came true" - welcome lecture

Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland

15:30 → 16:30 Prof. Alexandre Dazzi: "AFM–IR technique: History and principle"

Institut de Chimie Physique, Université Paris-Saclay, Orsay, France

16:30 → 17:00 Coffe break

17:00 → 18:00 Prof. Katarzyna Pogoda: "Atomic force microscopy in biological applications"

Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland

18:00 → 18:30 Dr. Karolina Chrabąszcz: "How to prepare cellular samples for AFM–IR investigations?"

Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland

18:30 → 20:30 Welcome cocktail

Tuesday 10 June

09:00 → 12:00 AFMIR measurements

09:00 → 12:00 FTIR measurements

09:00 → 12:00 biological panel

12:00 → 13:30 lunch

13:30 → 16:30 AFMIR measurements

13:30 → 16:30 FTIR measurements

13:30 → 16:30 system demonstrations

13:30 - 15:00 Labsoft "Nanomechanical properties of the biological samples studies by Bruker exclusive PeakForce QNM with Dimension IconIR"
15:00 - 16:30 Digital Surf "Mountains® software for AFM-IR data analysis"

16:30 → 17:00 coffee break

17:00 → 17:30 Dr. Jérémie Mathurin: "Nonlinear AFM–IR modes for biological samples"

Institut de Chimie Physique, Université Paris-Saclay, Orsay, France

17:30 → 18:00 Dr. Ewa Pięta: "Vibrational microspectroscopy: from fundamentals to applications"

Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland

18:00 → 19:30 Dr. Jakub Dybaś: "Basic Vibrational spectra processing and analysis for accurate presentation of datasets. Part I"

Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Kraków, Poland

Wednesday 11 June

09:00 → 12:00 AFMIR measurements

09:00 → 12:00 FTIR measurements

09:00 → 12:00 biological panel

12:00 → 13:30 lunch

13:30 → 16:30 AFMIR measurements

13:30 → 16:30 FTIR measurements

13:30 → 16:30 system demonstrations

13:30 - 15:00 Labsoft "Nanomechanical properties of the biological samples studies by Bruker exclusive PeakForce QNM with Dimension IconIR"
15:00 - 16:30 Digital Surf "Mountains® software for AFM-IR data analysis"

16:30 → 17:00 coffee break

17:00 → 17:30 Prof. Magdalena Oćwieja: "Plasmonic nanoparticle layers prepared through electrostatically driven self–assembly for nanopectroscopic applications"

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Krakow, Poland

17:30 → 18:00 Dr. Natalia Piergies: "Application of surface enhancement effect in the AFM–IR studies"

Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland

18:00 → 18:30 Dr. Dominika Święch: "Nano–spectroscopic approach to materials: AFM–IR studies of modified metalic surfaces"

AGH University of Krakow, Faculty of Foundry Engineering, Krakow, Poland

Thursday 12 June

09:00 → 10:00 Dr. Jakub Dybaś: "Basic Vibrational spectra processing and analysis for accurate presentation of datasets. Part II"

Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Kraków, Poland

10:15 → 12:15 Data analysis

12:15 → 13:30 lunch

13:30 → 15:30 Data analysis

17:00 → 19:00 Excursion

19:00 → 23:00 Conference dinner

Friday 13 June

10:00 → 11:00 Dr. David Pérez–Guaita: "Multimodal Spectroscopy: Data Fusion of Infrared spectroscopy and Complementary Techniques"

University of Valencia, Department of Analytical Chemistry, Valencia, Spain

11:00 → 11:30 coffee break

11:30 → 12:30 Prof. Ariane Deniset–Besseau: "Using AFM–IR technique in bioscience: Nanoscale insights from tissue to single biomolecules"

Institut de Chimie Physique, Université Paris-Saclay, Orsay, France

12:30 → 13:00 Results presentation group 1

13:00 → 14:30 lunch

14:30 → 15:00 Results presentation group 2

15:00 → 15:30 closing