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v3.3.1
Abstract:
During the lecture, I will focus on the comprehensive characterization of the adsorption phenomenon at the molecule/metal interface using surface-enhanced vibrational spectroscopy techniques (SERS and SEIRA). Furthermore, in order to approach the characterization of adsorption geometry even more deeply, another phenomenon, SEIRA at the nanoscale with two orthogonal polarization modulations will be applied. A few examples of the results obtained for anti-cancer drugs adsorbed on the Au nanoparticles surface will be thoroughly discussed. Emphasis will also be placed on the study of drug behavior on metal nanostructures and the interaction strength of the molecule with the metal nanosensor. Fundamental rules in surface-enhanced vibrational spectroscopy will be discussed, along with factors influencing the observed signal amplification. The main paths on how to determine the molecular structure of the tested compound, what factors affect the orientation of the molecule on the metal surface, and how to infer the strength of interaction with the studied substrate will be presented.
After understanding the interfacial behavior of drugs immobilized onto the metal surface, I will focus on assessing the nanoparticles penetration through the cell membrane. Then, the experiment focuses on monitoring the cell’s response to different concentrations of AuNPs and drug/AuNPs conjugate using FT-IR imaging will be demonstrated. The fundamental goal of the performed experiment was the application of FTIR spectroscopic imaging to study the cell’s response to drug/AuNPs conjugates to more accurately define the sensitivity of cancer cells to drugs. Finally, I will try to convince the audience that the simultaneous application of SERS, SEIRA, and polarization-modulated SEIRA in nanoscale has great potential for predicting the interfacial behavior of molecule-metal conjugates. Furthermore, I will discuss advantages connected with the application of spectroscopic measurements performed at the single-cell level, like FTIR imaging, to evaluate biochemical alterations of cellular components due to drug/nanoparticles treatment.